|
KMID : 1149220190270010045
|
|
Korean Journal of Oriental Medical Prescription
2019 Volume.27 No. 1 p.45 ~ p.52
|
|
|
Treatment of Cinnamomi Cortex combined with hyperthermia synergistically suppressed proliferation and induced apoptosis in U937 cell line
|
|
Ahn Chae-Ryeong
Park Sun-Hyang
Kim Hong-Jun
Jeong Ju-Hui
Baek Seung-Ho
|
|
|
Abstract
|
|
|
Objectives : Hyperthermia is a widely used therapeutic tool for cancer therapy and a well-known inducer of apoptosis. Although the Cinnamomi cortex (CC) is a potent anticancer agent for several human carcinomas, it is less potent in the human U937 cell line. To explore any enhancing effects of CC with hyperthermia induced apoptosis, this study investigated the combined effects and apoptotic mechanisms of hyperthermia and CC in U937 cells.
Methods : U937 cells were heat treated at 43ºC for 30 min with or without pre-treatment for 1h with CC and then incubated at 37ºC with 5% CO2. Cell viability was analyzed by MTT assay and Trypan blue assay. Morphological changes reflecting apoptosis were visualized under microscope. Synergy effect of CC combined with hyperthermia were calculated by Compusyn software. The expression of proteins related to apoptosis and signaling pathways was determined by western blotting.
Results : Hyperthermia with CC reduced cell viability and induced apoptosis. Combined hyperthermia and CC treatment markedly augmented apoptosis by upregulating proapoptotic proteins and suppressing antiapoptotic proteins, culminating in caspase-3 activation. Furthermore, the combined treatment, decreased the expression of in Bcl-2 family, cyclin D1, VEGF, MMP2 and MMP9 expression.
Conclusion : This study provides compelling evidence that hyperthermia, in combination with CC, is a promising therapeutic strategy for enhancement of apoptosis and suggests a promising therapeutic approach for cancer.
|
|
|
KEYWORD
|
|
|
Cinnamomi cortex, hyperthermia, synergy effect, apoptosis, leukemia cell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|